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Reagents & Instruments Table

SalivaDirect™ Reagents & Instruments
Total minimum reagent cost per sample $1.29-$4.37
Vendor Item Catalog number Price/Sample
Sample Processing (pick one of the listed options)
AmericanBio Proteinase K AB00925-00100 $0.13
ThermoFisher Scientific MagMAX Viral/Pathogen Proteinase K A42363 $0.16
New England Biolabs Proteinase K, Molecular Biology Grade P8107S $0.26
RT-qPCR Primers and Probes (pick one of the listed options)
Eurofins SalivaDirect primer probe set, 50-100 nmol 12YS-010YST $0.12
Integrated DNA technologies nCOV_N1 Forward Primer Aliquot, 50 nmol 10006821 $0.02
nCOV_N1 Forward Primer Aliquot, 100 nmol 10006830 $0.02
nCOV_N1 Reverse Primer Aliquot, 50 nmol 10006822 $0.02
nCOV_N1 Reverse Primer Aliquot, 100 nmol 10006831 $0.02
nCOV_N1 Probe Aliquot, 25 nmol 10006823 $0.04
nCOV_N1 Probe Aliquot, 50 nmol 10006832 $0.03
RNase P Forward Primer Aliquot, 50 nmol 10006827 $0.01
RNase P Forward Primer Aliquot, 100 nmol 10006836 $0.01
RNase P Reverse Primer Aliquot, 50 nmol 10006828 $0.01
RNase P Reverse Primer Aliquot, 100 nmol 10006837 $0.01
RP probe (Cy5-IBRQ) Custom $0.10
RP probe (ATTO657-IBRQ), 25 nmol 10007061 $0.09
RP probe (ATTO657-IBRQ), 50 nmol 10007062 $0.06
LGC Biosearch Technologies nCOV_N1 Forward Primer, 100 nmol nCoV-N1-F-100 $0.01
nCOV_N1 Forward Primer, 1000 nmol nCoV-N1-F-1000 $0.01
nCOV_N1 Reverse Primer, 100 nmol nCoV-N1-R-100 $0.01
nCOV_N1 Reverse Primer, 1000 nmol nCoV-N1-R-1000 $0.01
nCOV_N1 Probe, 25 nmol nCoV-N1-P-25 $0.04
nCOV_N1 Probe, 250 nmol nCoV-N1-P-250 $0.03
RNase P Forward Primer, 20 nmol RNP-F-20 <$0.01
RNase P Forward Primer, 100 nmol RNP-F-100 <$0.01
RNase P Forward Primer, 1000 nmol RNP-F-1000 <$0.01
RNase P Reverse Primer, 20 nmol RNP-R-20 <$0.01
RNase P Reverse Primer, 100 nmol RNP-R-100 <$0.01
RNase P Reverse Primer, 1000 nmol RNP-R-1000 <$0.01
RP probe, 25 nmol RNP-PQ670-25 $0.06
RP probe, 250 nmol RNP-PQ670-250 $0.03
RT-qPCR Kits (pick one of the listed options)
New England Biolabs Luna Universal Probe One-Step RT-qPCR Kit E3006S $0.75-$1.08
E3006L $0.75-$1.08
E3006X $0.75-$1.08
E3006E $0.75-$1.08
Bio-Rad Reliance One-Step Multiplex 12010176 $1.84-$2.11
RT-qPCR Supermix 12010220 $1.84-$2.11
12010221 $1.84-$2.11
ThermoFisher Scientific TaqPath 1-Step RT-qPCR Master Mix, GC A15299 $1.94-$2.06
A15300 $1.94-$2.06
Controls
Twist Bioscience Synthetic SARS-CoV-2 RNA Control 2 102024 <$0.01
Integrated DNA technologies Nuclease-free water 11-04-02-01 <$0.01
11-05-01-14 <$0.01
11-05-01-04 <$0.01
New England Biolabs Nuclease-free water B1500S <$0.01
B1500L <$0.01
RT-qPCR Instruments
Bio-Rad CFX96 Touch Real-Time PCR Detection System NA NA
ThermoFisher Scientific Applied Biosystems 7500 Fast Real-Time PCR System NA NA
Applied Biosystems 7500 Fast Dx Real-Time PCR System NA NA
ABI QuantStudio 5 Real-Time PCR System (96 well format) NA NA

Important Information about SalivaDirect™

SalivaDirect™has not been FDA cleared or approved. It has been authorized by the FDA under an emergency use authorization for use by authorized laboratories. The test has been authorized only for the detection of nucleic acid from SARS-CoV-2, not for any other viruses or pathogens. This test is only authorized for the duration of the declaration that circumstances exist justifying the authorization of emergency use of in vitro diagnostic tests for detection and/or diagnosis of COVID-19 under Section 564(b)(1) of the Act, 21 U.S.C. § 360bbb-3(b)(1), unless the authorization is terminated or revoked sooner.