Abhishek Jain

PhD, ME, BEngSci

Associate Research Scientist in Epidemiology (Environmental Health)

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Research Interests

Chemistry Techniques, Analytical; Analytical, Diagnostic and Therapeutic Techniques and Equipment

Public Health Interests

Biomarkers; Cancer; Environmental Health; Metabolism; Microbiome; Metabolomics

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Optimization of dried blood spot metabolomics

This was the first comprehensive study to optimize normalization approaches for accurate quantification of metabolites in DBS. The strength of this approach compared to other DBS metabolomics studies is the comparative analysis of five different normalization methods using multiple criteria. The criteria used for assessment include: the capability of a method to reduce intragroup variability, effect of the method on differential metabolic analysis, and the influence of the method on classification accuracy. This study constitutes an important advancement over previous studies, which have used only a single method and single criteria to normalize the DBS metabolome. Additionally, we introduced a novel normalization strategy and showed that SG can serve as a predictor of Hb. This relationship was also validated in an independent group

Overview of the workflow for comprehensive UPLC-MS/MS metabolomics analysis. Urine samples were collected before (V1) and after (V2) dietary intervention. Step 1 Sulfatase-based assay for selective identification of sulfated metabolites Step 2 Global analysis of the sulfated metabolome (sulfatome)

Comprehensive analysis of the sulfated metabolome as a signature for microbiome-host co-metabolism Discovery of 11 new metabolite markers for raspberries, soy milk, and flaxseeds. Structure validation of 130 sulfated metabolites (authentic standards, MS/MS fragmentation). 48 significantly upregulated sulfated metabolites upon dietary intervention

Comparison of two arylsulfatases for targeted mass spectrometric analysis of microbiota-derived metabolites

Targeted analysis of individual sulfated metabolome changes based on 235 compounds 1.The recombinant overexpressed arylsulfatase ASPC as a new tool for investigation of host-microbiota co-metabolism. 2. ASPC has similar substrate promiscuity and versatility than the commonly used arylsulfatase from Helix pomatia. 3. Arylsulfatases ASPC and extracted arylsulfatase from H. pomatia equally hydrolyzed more than 100 sulfated compounds. 4. 27 Metabolites of microbial origin were detected. 5. Hydrolysis of 21 metabolites solely by ASPC enhances the sulfated metabolome coverage.

Involvement of organic acids and amino acids in ameliorating Ni toxicity induced cell cycle dysregulation in Caulobacter crescentus: a metabolomics analysis

GC-MS metabolomics analysis of Ni(II) stressed C. crescentus showed an increased level of nine important metabolites including TCA cycle intermediates and amino acids. This indicates that changes in central carbon metabolism and nitrogen metabolism are linked with the disruption of cell division process. Addition of malic acid, citric acid, alanine, proline, and glutamine to 0.015 mM Ni(II)-treated C. crescentus restored its growth. Thus, the present work shows a protective effect of these organic acids and amino acids on Ni(II) toxicity. Metabolic stimulation through the PutA/GlnA pathway, accelerated degradation of CtrA, and Ni-chelation by organic acids or amino acids are some of the possible mechanisms suggested to be involved in enhancing C. crescentus’s tolerance. Our results shed light on the mechanism of increased Ni(II) tolerance in C. crescentus which may be useful in bioremediation strategies and synthetic biology applications such as the development of whole cell biosensor.

Similarities and differences in gut microbiome composition correlate with dietary patterns of Indian and Chinese adults

The interaction between diet and gut microbiota, and ultimately their link to health, has turned into the concentration of huge research. However, this relationship still needs to be fully characterized, particularly in case of the Asian population. We compared the fecal bacterial diversity and composition of healthy Indian and Chinese adults, ages 22–35 years, using next-generation sequencing analysis on IlluminaHiSeq 2500 platform. Our analysis revealed unique community structure, dominant Firmicutes, Actinobacteria and underrepresented Bacteroides, of Indian and Chinese gut bacteria. This community structure closely matched with the gut bacterial composition of the Russian population. Therefore, we hypothesized that enrichment of these bacterial clades is supported by high consumption of starch-rich diet such as rice, potato, refined grains. The dominance of genus Bifidobacterium due to carbohydrate-rich diet is another notable feature of this study. Moreover, Indian gut bacteria are significantly represented by Bacteroidetes and Prevotella, in contrast to Chinese, which could be associated with whole grains and plant based vegetarian diet of Indians. The gut bacterial population of Indian adults were as diverse as Chinese adults but a significant difference was noticed in gut bacterial composition and relative abundance between two populations.

Selected Publications

Multi-omic analysis reveals metabolic pathways that characterize right-sided colon cancer liver metastasisMorris M, Jain A, Sun B, Kurbatov V, Muca E, Zeng Z, Jin Y, Roper J, Lu J, Paty P, Johnson C, Khan S. Multi-omic analysis reveals metabolic pathways that characterize right-sided colon cancer liver metastasis. Cancer Letters 2023, 574: 216384. PMID: 37716465, PMCID: PMC10620771, DOI: 10.1016/j.canlet.2023.216384.
Urinary Paraben Concentrations and Associations with the Periconceptional Urinary Metabolome: Untargeted and Targeted Metabolomics Analyses of Participants from the Early Pregnancy StudyVollmar A, Rattray N, Cai Y, Jain A, Yan H, Deziel N, Calafat A, Wilcox A, Jukic A, Johnson C. Urinary Paraben Concentrations and Associations with the Periconceptional Urinary Metabolome: Untargeted and Targeted Metabolomics Analyses of Participants from the Early Pregnancy Study. Environmental Health Perspectives 2023, 131: 097006. PMID: 37702489, PMCID: PMC10498870, DOI: 10.1289/ehp12125.
Discovery of decreased ferroptosis in male colorectal cancer patients with KRAS mutations.Yan H, Talty R, Jain A, Cai Y, Zheng J, Shen X, Muca E, Paty PB, Bosenberg MW, Khan SA, Johnson CH. Discovery of decreased ferroptosis in male colorectal cancer patients with KRAS mutations. BioRxiv 2023 PMID: 36909561, DOI: 10.1101/2023.02.28.530478.
Hemoglobin normalization outperforms other methods for standardizing dried blood spot metabolomics: A comparative studyJain A, Morris M, Lin EZ, Khan SA, Ma X, Deziel NC, Godri Pollitt KJ, Johnson CH. Hemoglobin normalization outperforms other methods for standardizing dried blood spot metabolomics: A comparative study. The Science Of The Total Environment 2022, 854: 158716. PMID: 36113793, DOI: 10.1016/j.scitotenv.2022.158716.
Asparagine, colorectal cancer, and the role of sex, genes, microbes, and diet: A narrative reviewShen X, Jain A, Aladelokun O, Yan H, Gilbride A, Ferrucci LM, Lu L, Khan SA, Johnson CH. Asparagine, colorectal cancer, and the role of sex, genes, microbes, and diet: A narrative review. Frontiers In Molecular Biosciences 2022, 9: 958666. PMID: 36090030, PMCID: PMC9453556, DOI: 10.3389/fmolb.2022.958666.
Comparison of two arylsulfatases for targeted mass spectrometric analysis of microbiota-derived metabolites.Jain A, Correia MSP, Meistermann H, Vujasinovic M, Löhr JM, Globisch D. Comparison of two arylsulfatases for targeted mass spectrometric analysis of microbiota-derived metabolites. Journal Of Pharmaceutical And Biomedical Analysis 2021, 195: 113818. PMID: 33342568, DOI: 10.1016/j.jpba.2020.113818.
Comparative dietary sulfated metabolome analysis reveals unknown metabolic interactions of the gut microbiome and the human host.Correia MSP, Jain A, Alotaibi W, Young Tie Yang P, Rodriguez-Mateos A, Globisch D. Comparative dietary sulfated metabolome analysis reveals unknown metabolic interactions of the gut microbiome and the human host. Free Radical Biology & Medicine 2020, 160: 745-754. PMID: 32927015, DOI: 10.1016/j.freeradbiomed.2020.09.006.
Rapid Preparation of a Large Sulfated Metabolite Library for Structure Validation in Human Samples.Correia MSP, Lin W, Aria AJ, Jain A, Globisch D. Rapid Preparation of a Large Sulfated Metabolite Library for Structure Validation in Human Samples. Metabolites 2020, 10 PMID: 33081284, PMCID: PMC7603051, DOI: 10.3390/metabo10100415.
An untargeted fecal and urine metabolomics analysis of the interplay between the gut microbiome, diet and human metabolism in Indian and Chinese adults.Jain A, Li XH, Chen WN. An untargeted fecal and urine metabolomics analysis of the interplay between the gut microbiome, diet and human metabolism in Indian and Chinese adults. Scientific Reports 2019, 9: 9191. PMID: 31235863, PMCID: PMC6591403, DOI: 10.1038/s41598-019-45640-y.
Similarities and differences in gut microbiome composition correlate with dietary patterns of Indian and Chinese adults.Jain A, Li XH, Chen WN. Similarities and differences in gut microbiome composition correlate with dietary patterns of Indian and Chinese adults. AMB Express 2018, 8: 104. PMID: 29936607, PMCID: PMC6015586, DOI: 10.1186/s13568-018-0632-1.
Involvement of organic acids and amino acids in ameliorating Ni(II) toxicity induced cell cycle dysregulation in Caulobacter crescentus: a metabolomics analysis.Jain A, Chen WN. Involvement of organic acids and amino acids in ameliorating Ni(II) toxicity induced cell cycle dysregulation in Caulobacter crescentus: a metabolomics analysis. Applied Microbiology And Biotechnology 2018, 102: 4563-4575. PMID: 29616314, DOI: 10.1007/s00253-018-8938-0.

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